Kleihauer,Fetal Hgb/Interp

Test Code

Alias/See Also
Fetal Stain

CPT Codes

Preferred Specimen


Minimum Volume
1 ml


Maternal blood should be collected as soon after delivery as possible.

Transport Container


Transport Temperature
Room Temp, or 2-8 degrees C

Specimen Stability
48 hours at 4-8 degrees C; 24 hours at Room Temp

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Cord Blood or any sample >24 hours old. clotted samples or fibrin strands are also rejected

Manual with Acid Elution technique

Setup Schedule

Daily on Receipt

Report Available
Upon Completion of Analysis

lipemia, icterus, cold agglutinin

Reference Range


Clinical Significance

Hemoglobin F is a normal hemoglobin in infants. HgbF may also appear elevated in obstetric patients in which a large feto-maternal bleed is suspected. In such cases, a dose of Rhogam may be indicated, in appropriate patients, to suppress the formation of antibodies by the mother. Intra-uterine transfusion is another occasion when Kleihauer stain may be requested. Pre and post samples are collected to look for a drop in results indicating the transfusion was successful.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.