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Fluid Smear/Gram Stain
Test CodeFLSM
CPT Codes
87205
Preferred Specimen
Sterile body fluid including CSF
Minimum Volume
CSF-2 ml, Other Sterile body fluid-10 ml
Instructions
Collect specimen aseptically; transport in a sterile container. **Sterile body fluids may be directly inoculated into Bactec Plus Blood Culture Bottles, 8-10 mls per bottles. If fluid is received in Bactec Bottles a gram stain will not be performed unless additional fluid is recieved for processing of gram stain.
Transport Container
Sterile Container
Transport Temperature
CSF-RT,immediately. Other Sterile Body Fluid-Store at RT for 2 hr, then 2-8 C.
Specimen Stability
2-8 C for up to 24 hrs.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improperly labelled; Improper storage/transport
Methodology
Microscopic examination; gram stain
Setup Schedule
Daily upon receipt
Report Available
Upon Completion of Analysis
Reference Range
No Bacteria Seen
Clinical Significance
The gram stain is a critical test for the rapid, presumptive diagnosis of infectious agents and also serves to assess the quality of clinical specimens.