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Bilirubin, Direct
Test CodeDBILI
CPT Codes
82248
Preferred Specimen
Serum (gold top) tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Lithium Heparin Plasma (green top) tube
Instructions
Protect specimen from prolonged exposure to light using a brown paper sack, aluminum foil, or a darkly colored tube.
Transport Container
Serum (gold top) tube or Lithium Heparin Plasma (green top) tube. Protect from light using brown paper sack, aluminum foil, or brown plastic specimen tube.
Transport Temperature
Room Temperature or Refrigeration
Specimen Stability
Room Temperature - 4 hours; Refrigerated - 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Quantity Not Sufficient, EDTA (purple top tube) contamination, IV contamination
Methodology
Colorimetric
Setup Schedule
Daily upon receipt
Report Available
Upon completion of analysis
Reference Range
0 - 0.3 mg/dL
Clinical Significance
Direct bilirubin is used for evaluating liver and biliary diseases. An increased direct bilirubin occurs in both intrahepatic and extrahepatic biliary tract obstructions. Hepatocellular causes of elevation include hepatitis, cirrhosis, and advanced neoplastic states. Direct bilirubin is also increased in Dubin-Johnson syndrome and in Rotor syndrome.