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FISH, DDIT3 (CHOP) 12q13.3
Test Code33942
CPT Codes
88271 (x2), 88275
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Minimum Volume
3 slides
Other Acceptable Specimens
5 slides unstained 5 micron slides on charged (+) slides
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Send specimen to testing lab for determination. Do not freeze. Do not reject.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Send specimen to testing lab for determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Acceptable
Refrigerated: Preferred
Frozen: Unacceptable
Refrigerated: Preferred
Frozen: Unacceptable
Methodology
Fluorescence in situ Hybridization (FISH)
Setup Schedule
Set up: Daily; Report available: 5-7 days
Reference Range
See Laboratory Report
Clinical Significance
The DDIT3 (CHOP) dual color break apart probe set will detect rearrangements of chromosome region 12q13.1-12q13.2 from cultured cells and FFPE sections. Liposarcoma is the most common soft tissue malignancy in adults accounting for at least 20% of all sarcomas in this age group. DDIT3 is implicated in two translocations associated with myxoid- round cell liposarcoma: t(12;16)(q13;p11) and t(12;22) (q13;q12) creating FUS/DDIT3 and EWS/DDIT3 chimeric genes, respectively. Also DDIT3 may be involved in translocations with variant chromosome regions. The fusion genes generated as a result of chromosomal rearrangements are used to monitor diagnosis and treatment.
