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FISH, IGL-BA 22q11.22
Test Code33547
CPT Codes
88271 (x2), 88275
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block, or
5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) or EDTA (lavender-top) tube
5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) or EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1 mL bone marrow • 3 slides
Other Acceptable Specimens
5 slides unstained 5 micron slides on charged (+) slides, or bone marrow transport tube (available upon request)
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
FFPE and Slides
Room temperature: Acceptable
Refrigerated: Preferred
Frozen: Unacceptable
Whole blood and bone marrow
Room temperature: Preferred
Refrigerated: Acceptable
Frozen: Unacceptable
Room temperature: Acceptable
Refrigerated: Preferred
Frozen: Unacceptable
Whole blood and bone marrow
Room temperature: Preferred
Refrigerated: Acceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
See instructions
Methodology
Fluorescence In Situ Hybridization (FISH)
Setup Schedule
Set up: Daily; Report available: 5-7 days
Reference Range
See Laboratory Report
Clinical Significance
The cytogenetics department has FISH panels for chronic lymphocytic leukemia, plasma cell myeloma, high grade lymphoma and acute lymphoblastic leukemia. The probes in these panels contain probes that determine rearrangements of gene regions with IGH. When IGH is intact in these analyses and there is evidence of a possible variant translocation for a disease-associated gene region, it is important to determine if there is a translocation involving one of the immunoglobulin light chain gene regions. Translocations involving the immunoglobulin loci are recurring events in various subtypes of B-cell lymphomas. In addition to translocations involving the IGH locus, variant translocations have been described in 5-10% of B-cell neoplasms involving either the immunoglobulin kappa (IGK) light chain or the lambda light chain (IGL). The best known translocations involving IG light chain loci are the variant Burkitt's translocations t(2;8)(p12;q24) and t(8;22)(q24;q11) present in up to 25% of all Burkitt's lymphomas. Other translocations involve the BCL6 oncogene: the t(2;3)(p12;q27) and t(3;22)(q27;q11) and BCL2 locus: t(2;18)(p12;q21) and t(18;22)(q21;q11) in F.L. Translocations involving the IG light chain loci usually lead to breakage within the joining region of the respective locus. These IGH and variant rearrangements may occur in combination in "double hit" and "triple hit" lymphomas.
The IGL break apart FISH probe allows for definitive identification involving the rearrangement of the immunoglobulin light chain locus (IGL) located at chromosome band 22q11.22 in suspension cultures and FFPE sections. This probe is useful for identifying variant translocations and for identifying rearrangements in complex karyotypes.
The IGL break apart FISH probe allows for definitive identification involving the rearrangement of the immunoglobulin light chain locus (IGL) located at chromosome band 22q11.22 in suspension cultures and FFPE sections. This probe is useful for identifying variant translocations and for identifying rearrangements in complex karyotypes.
