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FISH, RET
Test Code33485
CPT Codes
88271 (x2), 88274
Includes
If results are not possible from the submitted specimen, the test order will be cancelled and replaced by non-orderable test Cytogenetics Communication.
Preferred Specimen
Formalin-fixed paraffin-embedded tissue block
Minimum Volume
3 slides
Other Acceptable Specimens
5 slides, unstained (5 microns) on positively charged (+) slides
Instructions
Do not reject specimens, send to laboratory for screening.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Send specimen to testing lab for determination. Do not freeze. Do not reject.
Paraffin block is the preferred sample type. Sample source and block ID are required on the requisition form and a pathology report must be submitted.
Send specimen to testing lab for determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Acceptable
Refrigerated: Preferred
Frozen: Unacceptable
Refrigerated: Preferred
Frozen: Unacceptable
Methodology
Fluorescence in situ Hybridization (FISH)
Setup Schedule
Set up: Daily; Report available: 5-7 days
Reference Range
See Laboratory Report
Clinical Significance
The RET red/green FISH probe mixture allows for definitive identification of the rearrangement in FFPE tumor specimens. RET rearrangements have been identified in 1-2% of lung adenocarcinomas (non-small cell lung cancer), especially in a younger population (<30) which are negative for EGFR, KRAS, ROS1, and ALK abnormalities. Lung cancer studies have shown these patients responding to treatments with tyrosine kinase inhibitors (TKI) such as cabozantinib. Also, RET rearrangements have been found in radiation induced papillary and medullary thyroid carcinomas.
