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NPM1 Mutation Detection by RT-PCR, Quantitative
Test Code13058
CPT Codes
81310<br>Restricted Client Code
Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1 mL bone marrow
Other Acceptable Specimens
3 mL bone marrow collected in an EDTA (lavender-top) tube
Instructions
Refrigerate immediately. Specimens must be received within 48 hours of collection due to lability of RNA.
Separate specimens must be submitted when multiple tests are ordered.
Separate specimens must be submitted when multiple tests are ordered.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: Unacceptable
Refrigerated: 48 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum, plasma, ambient or frozen bone marrow or ambient or frozen whole blood, CSF, or FFPE tissue • Specimens collected in anticoagulants other than EDTA • Severely hemolyzed or clotted specimens • Room temperature whole blood and room temperature bone marrow specimens past 7 days will be canceled • Refrigerated whole blood or bone marrow past 7 days will be canceled
Methodology
Quantitative Reverse Transcription Polymerase Chain Reaction
Setup Schedule
Set up: Varies; Report available: 5-9 days
Reference Range
See Laboratory Report
Clinical Significance
Use to detect and quantitate NPM1 mutant transcripts (type A, B and D). Use to monitor for minimal residual disease and assess the risk of disease relapse.
