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Phosphatidylinositol Antibody (IgA)
Test Code10517
CPT Codes
83520
Preferred Specimen
1 mL serum
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: 3.2% sodium citrate (light blue-top) tube
Instructions
Serum: Collect the blood in a serum separator tube (SST) (red-top Vacutainers or similar). Centrifuge the sample at 1,300 g for 15 min (after 30 min of drawing the blood). Transfer the serum to a plastic tube (approximately 1 mL of serum is necessary for each test). Label the specimen properly. If sample is not to be shipped immediately, the specimen should be stored at 2-8° C. If specimens are to be stored for more than 4 hours, they should be frozen at -20° C or below.
Plasma: Collect the blood in anti-coagulated citrate tubes (blue-top or light blue-top Vacutainer or similar Na Citrate 3.2%). Plasma must be separated from whole blood by centrifugation for 15 minutes at 2,000-2,500 g. After centrifugation, remove only the upper 2/3 parts of the supernatant and transfer to a plastic tube (approximately 1 mL of plasma is necessary for each test). The centrifugation steps are crucial since complete removal of platelets from the plasma is important to obtain accurate results. Repeating the centrifugation and transferring the upper 2/3 parts of the supernatant to a third plastic tube may be advisable to minimize platelet contamination. Label the specimen properly. If sample is not to be shipped immediately, the specimen should be stored at 2-8° C. If specimens are to be stored for more than 4 hours, they should be frozen at -20° C or below.
Shipping: Place the tube(s) or vials(s) in a secondary container (a plastic biohazard bag with an absorbent material to avoid leakage of the specimen). Always mail the specimen with the corresponding completed requisition form. Place the secondary container preferably in a Styrofoam box with cool packs. Alternatively, the specimen may be shipped frozen with dry ice. Include the filled Requisition Form with each specimen. Always ship specimens via overnight courier.
We provide upon request a detailed Specimen Requirement sheet. Please request it via email to apls@aplslab.com or by fax to 844-721-8193.
SHIP TO: ANTIPHOSPHOLIPID STND LAB
702 9th ST N
TEXAS CITY, TX 77590
PHONE: 409-539-7588
Plasma: Collect the blood in anti-coagulated citrate tubes (blue-top or light blue-top Vacutainer or similar Na Citrate 3.2%). Plasma must be separated from whole blood by centrifugation for 15 minutes at 2,000-2,500 g. After centrifugation, remove only the upper 2/3 parts of the supernatant and transfer to a plastic tube (approximately 1 mL of plasma is necessary for each test). The centrifugation steps are crucial since complete removal of platelets from the plasma is important to obtain accurate results. Repeating the centrifugation and transferring the upper 2/3 parts of the supernatant to a third plastic tube may be advisable to minimize platelet contamination. Label the specimen properly. If sample is not to be shipped immediately, the specimen should be stored at 2-8° C. If specimens are to be stored for more than 4 hours, they should be frozen at -20° C or below.
Shipping: Place the tube(s) or vials(s) in a secondary container (a plastic biohazard bag with an absorbent material to avoid leakage of the specimen). Always mail the specimen with the corresponding completed requisition form. Place the secondary container preferably in a Styrofoam box with cool packs. Alternatively, the specimen may be shipped frozen with dry ice. Include the filled Requisition Form with each specimen. Always ship specimens via overnight courier.
We provide upon request a detailed Specimen Requirement sheet. Please request it via email to apls@aplslab.com or by fax to 844-721-8193.
SHIP TO: ANTIPHOSPHOLIPID STND LAB
702 9th ST N
TEXAS CITY, TX 77590
PHONE: 409-539-7588
Transport Container
Transport tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 1 year
Refrigerated: 14 days
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Icteric • Received room temperature
Methodology
Enzyme Linked Immunosorbent Assay (ELISA)
Setup Schedule
Set up: Wed, Fri; Report available: 3-5 days
Reference Range
See Laboratory Report
Clinical Significance
Antiphospholipid Syndrome (APS) is a disorder complicated by recurrent arterial and venous thrombosis (e.g., cerebrovascular accident, myocardial infarction, pulmonary thromboembolism) and/or recurrent pregnancy losses. Patients have positive lupus anticoagulant or anticardiolipin tests attributable to antiphospholipid (aPL) antibodies.
Whether these antibodies are epiphenomena or play a causative role in the disorder is uncertain, but this distinction must be made to better manage affected patients. A diagnosis of APS is based on positive aCL and or LA tests accompanied by one or more of the clinical symptoms.
Whether these antibodies are epiphenomena or play a causative role in the disorder is uncertain, but this distinction must be made to better manage affected patients. A diagnosis of APS is based on positive aCL and or LA tests accompanied by one or more of the clinical symptoms.
