FISH, MYB, Deletion 6q23, TC

Test Code

12643

CPT Codes
88271, 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • Bone marrow in transport medium • 5x5mm lymph node submitted in culture transport medium

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

See Instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

100-300 interphase cells are microscopically analyzed for probe signal patterns, indicating the copy number for the MYB region of chromosome 6q23.

Chromosome 6q deletion is a frequent abnormality in lymphoid malignancies, including acute lymphoblastic leukemia, chronic lymphoid disorders, non-Hodgkin lymphoma, and multiple myeloma. It may be the sole clonal abnormality in as many as 30% of cases. Deletions may be small and difficult to resolve, using conventional chromosome analysis. Deletion of MYB may be associated with loss of a recessive tumor suppressor gene and may be an initial step in leukemogenesis.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.