FISH, TCL1, 14q32.1, TC

Test Code

12642

CPT Codes
88271 x 2, 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • Bone marrow in transport medium • 5 x5mm tumor tissue in formalin-fixed paraffin-embedded tissue block

Instructions

Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.

1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin (green-top, dark/royal blue-top, tan-top) tube. Formalin fixed paraffin embedded tumor biopsy.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

⁠⁠⁠⁠⁠⁠⁠See Instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

The proto-oncogene TCL1, located at 14q32.1, is overexpressed in a variety of lymphoid neoplasms, including T-cell prolymphocytic leukemia (T-PLL), T-cell acute lymphoblastic leukemia/lymphoma (T-ALL/LBL), Epstein-Barr virus (EBV)-infected B-cell lymphomas, and AIDS-related lymphomas. The overexpression of TCL1 usually results from juxtaposition to a T-cell receptor locus via a translocation or inversion.

100-300 interphase cells are microscopically analyzed for probe signal patterns indicating rearrangement of 14q32.1.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.