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FISH, CLL, CEP 12, Trisomy 12, TC
Test Code12637
CPT Codes
88271, 88275<br>Restricted Client Code - Tech Only
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Other Acceptable Specimens
Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • 5 x5mm fresh lymph node collected in culture transport medium
Instructions
Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
See Instructions
Methodology
Fluorescence in situ Hybridization
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Clinical Significance
This test is performed in addition to routine chromosome analysis. FISH (fluorescence in situ hybridization) analysis is performed on 100 or more interphase nuclei from peripheral blood, bone marrow or lymph node to identify and enumerate chromosome 12 using a DNA probe specific for the centromeric region. Trisomy 12 is one of the most commonly reported chromosome aberrations in B-cell chronic lymphocytic leukemia (CLL) and is of prognostic importance. FISH analysis detects trisomy 12 in non-dividing cells which cannot be identified with routine cytogenetic studies.