FISH, ALL/NHL, MYC BA, 8q24 Rearrangement, TC

Test Code

12626

CPT Codes
88271 (x2), 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

5 x 5mm lymph node collected in a sterile container in Hanks' Ringers solution or culture medium with antibiotics

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

3 mL bone marrow or 5 mL whole blood collected in: sodium heparin (green-top) tube, or sodium heparin, lead-free (tan-top) tube • 5 x 5mm lymph node tissue in formalin-fixed paraffin-embedded tissue block • 5 x5mm fresh (unfixed) lymph node tissue

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

For bone marrow (1-3 mL) or whole blood (3-5 mL), use a green-top tube with sodium heparin. For lymph node biopsy, 5 x 5 mm in sterile container in Hanks' Ringers solution or culture medium with antibiotics. Transportation medium available upon request.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

See Instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

This fluorescence in situ hybridization (FISH) test is used to identify rearrangements of the C-MYC region located on chromosome band 8q24 using the dual color breakapart (BA) DNA probe. This rearrangement is observed in a majority of Burkitt lymphomas.

100-200 interphase nuclei are examined microscopically for the suspected gene rearrangement. This test is useful for evaluation of suboptimal specimens or when a variant or cryptic rearrangement is suspected.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.