FISH, Burkitt's/NHL/ALL, IGH/MYC, t(8,14), TC

Test Code

12624

CPT Codes
88271 (x3), 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

5 mL whole blood or 3 mL bone marrow collected in sodium heparin (green-top or royal blue-top) tube, or sodium heparin, lead-free (tan-top) tube, or
5 X 5 mm fresh tissue collected in culture transport media or sterile container with Hanks' or Ringers solution, or
Formalin-fixed paraffin-embedded tissue

Minimum Volume

1 mL whole blood • 1 mL bone marrow

Instructions

Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.

3-5 mL whole blood or 3 mL bone marrow collected in sodium heparin tube, 5 X 5 mm Fresh tissue in sterile container, Formalin Fixed Paraffin Embedded Tissue.

Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

See Instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

Fluorescence in situ hybridization (FISH) test is performed to detect translocation between chromosome bands 8q24 (MYC) and 14q32 (IGH) associated with acute lymphocytic leukemia (ALL) and non Hodgkin's lymphoma (NHL) Burkitt's type. 100-200 interphase nuclei are examined microscopically for the presence of fusion signals for the suspected gene rearrangement. This test is useful for evaluation of suboptimal specimens or when a variant or cryptic rearrangement is suspected.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.