FISH, D13S319, Deletion 13q14.3, TC

Test Code

12622

CPT Codes
88271 (x2), 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin, lead-free (tan-top) tube • 5 x5mm fresh lymph node collected in Hanks' or Ringer's solution

Instructions

Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.

Submit 1 to 3 mL of bone marrow or 3 to 5 mL whole blood in a sodium heparin (green-top) tube.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

See Instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

This test is performed in addition to G-banding chromosome analysis tests for bone marrow, hematologic blood, or lymph node.

Deletion of chromosome 13q14.3 occurs in multiple myeloma, non Hodgkin?s lymphoma, chronic lymphocytic leukemia, myeloproliferative disorders, myelodysplastic syndrome, and acute myelogenous leukemia. The D13S319 Fluorescence In Situ Hybridization (FISH) probe is useful in detecting deletions in a higher percentage of cells than by routine cytogenetic studies. The probe can be used to evaluate complex karyotypes for loss of this critical gene region.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.