FISH, p53, Deletion 17p13.1, TC

Test Code

12619

CPT Codes
88271 (x2), 88275<br>Restricted Client Code - Tech Only

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow
3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • Bone marrow submitted in culture transport media • 5 x5mm fresh lymph node tissue submitted in culture transport media • 5 x5mm fresh biopsy submitted in culture transport media

Instructions

Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.

Please specify if this test is run for plasma cell neoplasms.

Submit 1 to 3 mL of bone marrow or 3 to 5 mL whole blood in a sodium heparin (green-top) tube. Bone marrow transport medium is available upon request.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

See Instructions

Methodology
Fluorescence In Situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Clinical Significance

Loss of the p53 gene, located in chromosome 17p13.1, is a common occurrence in neoplastic disorders, including hematologic disorders. FISH studies for deletion p53 are performed in addition to G-banding analysis of bone marrow, hematologic blood, lymph nodes, and solid tumors. This probe may be useful in detecting loss of p53 in complex karyotypes. Deletion of p53 is an adverse prognostic marker in CLL and follicular lymphoma.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.