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Steroid Panel, Comprehensive
Test Code90392
CPT Codes
82634, 83498, 84143, 82542, 82157, 82528, 82533, 82626, 82633, 84140, 84144, 84403
Includes
11-Deoxycortisol
17-Hydroxyprogesterone
17-Hydroxypregnenolone
18-Hydroxycorticosterone and Cortisone
Androstenedione
Corticosterone
Cortisol
DHEA, Unconjugated
Deoxycorticosterone
Pregnenolone
Progesterone
Testosterone, Total, LC/MS/MS
17-Hydroxyprogesterone
17-Hydroxypregnenolone
18-Hydroxycorticosterone and Cortisone
Androstenedione
Corticosterone
Cortisol
DHEA, Unconjugated
Deoxycorticosterone
Pregnenolone
Progesterone
Testosterone, Total, LC/MS/MS
Preferred Specimen
0.5 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.25 mL
Instructions
Collect blood in a Vacutainer with no additives. Allow blood to clot (30 minutes) at room temperature, 18°C to 25°C. Centrifuge and separate the serum from the cells. Freeze as soon as possible.
Transport Container
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: 28 days
Refrigerated: 72 hours
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Moderate hemolysis • Grossly lipemic • Grossly icteric • Serum separator tube (SST) • Plasma
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Set up: Sun, Tues; Report available: 5-10 days
Reference Range
See individual tests
Clinical Significance
This panel identifies all major adrenal steroid hormones and facilitates the diagnosis of the four enzymatic defects associated with congenital adrenal hyperplasia (CAH). All steroids are determined in a single assay by LCMSMS using as little as 0.1 mL serum.
