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5-HIAA (5-Hydroxyindoleacetic acid), Plasma
Test Code94149
CPT Codes
83497<br>Limited Access Code
Preferred Specimen
10 mL plasma collected in an EDTA (lavender-top) tube
Patient Preparation
Patient should fast overnight prior to collection of specimens
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma submitted in an ISI Z-tube™
Instructions
Collect using EDTA "purple top" plasma tube or 10 mL blood directly into ISI?s Z-tube Preservative and separate as soon as possible. Freeze plasma immediately after separation. Special Z-tube Preservatives are available from ISI. Minimum specimen size is 0.5 mL.
Note: One Z-tube may be shared for up to three tests. After centrifuging tube, aliquot 1 mL per test in separate vials, mark with name of test and freeze.
Important Precaution:
Specimens for this assay must be collected using EDTA "purple top" or ISI?s Z-tube. Specimens must be shipped frozen; specimens are not stable refrigerated or ambient. No substitute preservatives are acceptable.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 1 hour
Refrigerated: 7 days
Frozen: 4 months
Refrigerated: 7 days
Frozen: 4 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
No other specimens are acceptable • Not collected in Z-tube™ Preservative • Refrigerated specimens • Room temperature specimens
Methodology
Gas Chromatogragphy/Mass Spectrophotometry (GC-MS/MS)
Setup Schedule
Set up: Varies; Report available: 4-6 days
Reference Range
Up to 22 ng/mL
Clinical Significance
The ISI plasma 5-HIAA assay correlates well with the 24-hour urinary 5-HIAA assays. This test has been clinically validated for nets patients who previously relied on the 24-hour urinary 5-HIAA. The plasma 5-HIAA saves time, alleviates the need to collect urine in a container for 24 hours and provides equivalent clinical information.
