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Prothrombin Fragment 1.2
Test Code37674
CPT Codes
83520
Preferred Specimen
1 mL frozen platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 4 hours
Refrigerated: 4 hours
Frozen: 30 days
Refrigerated: 4 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Received refrigerated
Methodology
Enzyme Immunoassay (EIA)
Setup Schedule
Set up: Wed; Report available: 4 days
Reference Range
| ADULT | 41-372 pmol/L |
Clinical Significance
When prothrombin is converted to thrombin, the prothrombin fragment 1.2 is released from prothrombin. The test is useful to assess ongoing coagulation activation as may occur in thrombosis or DIC.
