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| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Protein, Total and Protein Electrophoresis
Test Code747
CPT Codes
84155, 84165
Preferred Specimen
2 mL serum
Patient Preparation
Overnight fasting is preferred
Minimum Volume
1 mL
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 5 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Plasma
Methodology
Capillary Zone Electrophoresis (CZE) • Spectrophotometry (SP)
Setup Schedule
Set up: Mon-Sat; Report available: 2-4 days
Reference Range
Protein, Total, Serum
Protein Electrophoresis
| Male (g/dL) | Female (g/dL) | ||||
| <1 Month | 4.1-6.3 | 4.2-6.2 | |||
| 1-5 Months | 4.7-6.7 | 4.4-6.6 | |||
| 6-11 Months | 5.5-7.0 | 5.6-7.9 | |||
| 1-19 Years | 6.3-8.2 | 6.3-8.2 | |||
| ≥20 Years | 6.1-8.1 | 6.1-8.1 |
Protein Electrophoresis
| Albumin | 3.8-4.8 g/dL |
| Alpha-1 Globulin | 0.2-0.3 g/dL |
| Alpha-2 Globulin | 0.5-0.9 g/dL |
| Beta-1 Globulin | 0.4-0.6 g/dL |
| Beta-2 Globulin | 0.2-0.5 g/dL |
| Gamma Globulin | 0.8-1.7 g/dL |
| Abnormal Protein Band 1 | None detected |
| Abnormal Protein Band 2 | None detected |
| Abnormal Protein Band 3 | None detected |
Clinical Significance
Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).
