Phospho-histone H3, IHC without Interpretation

Test Code

16257

CPT Codes
88342-TC

Preferred Specimen

Formalin-fixed, paraffin-embedded tissue block

Other Acceptable Specimens

Minimum: 3 unstained, positively charged slides

Transport Container

IHC specimen transport kit

Transport Temperature

Room temperature

Specimen Stability

Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable

Methodology
Immunohistochemistry (IHC)

Setup Schedule

Set up: Mon-Fri; Report available: Next day

Reference Range

See Laboratory Report

Clinical Significance

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (9). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.