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Herpes Virus 7 DNA, Qualitative Real-Time PCR [19769X]
Test Code19769
CPT Codes
87798<br /> **This test is not available for New York patient testing.**
Preferred Specimen
1 mL serum
Minimum Volume
0.3 mL
Other Acceptable Specimens
Plasma or whole blood collected in: EDTA (lavender-top), or acid citrate dextrose ACD-A (yellow-top) tube
Instructions
Serum: Collect blood in sterile tubes with no anticoagulants. Allow blood to clot at room temperature and separate serum from cells within 2 hours of collection. Transfer serum to sterile, plastic, screw-capped, aliquot tubes.
Plasma: Collect blood in sterile tubes containing EDTA (lavender-top) or ACD-A (yellow-top) as an anticoagulant. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped, aliquot tubes. Do not clarify plasma by filtration or further centrifugation.
Avoid repeated freezing and thawing of specimen. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA (lavender-top) or ACD-A (yellow-top) as an anticoagulant. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped, aliquot tubes. Do not clarify plasma by filtration or further centrifugation.
Avoid repeated freezing and thawing of specimen. Do not freeze whole blood.
Transport Container
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 30 days; (Whole blood: Unacceptable)
Refrigerated: 7 days
Frozen: 30 days; (Whole blood: Unacceptable)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen whole blood
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
FDA Status
This test is performed pursuant to a license agreement with Roche Molecular Systems, Inc.
Setup Schedule
Set up: Daily a.m.; Report available: 1 day
Reference Range
Not detected
Clinical Significance
This test is used in the diagnosis of human herpesvirus 7
infection. HHV-7 is closely related to HHV-6 and CMV,
and can cause reactivation disease in transplant patients
or other immune-compromised individuals. PCR may detect
infection earlier than immunological methods and provides
more rapid results than culture, but does not provide
information on past exposure or immunity.
infection. HHV-7 is closely related to HHV-6 and CMV,
and can cause reactivation disease in transplant patients
or other immune-compromised individuals. PCR may detect
infection earlier than immunological methods and provides
more rapid results than culture, but does not provide
information on past exposure or immunity.
