Free Progesterone Profile

Test Code

15072

CPT Codes
84144 (x2)<br>This test is not available for New York patient testing

Preferred Specimen

3 mL frozen serum

Patient Preparation
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medication, if possible, for at least 48 hours prior to collection of specimen.

Minimum Volume

2 mL

Other Acceptable Specimens

Plasma collected in an EDTA (lavender-top) tube

Instructions

3 mL serum or EDTA plasma should be collected and separated and frozen as soon as possible.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 90 days

Methodology
Radioimmunoassay following ultrafiltration of specimens

Setup Schedule

Set up: Mon-Fri; Report available: 12 days

Reference Range

Free Progesterone
Male:	5-20 ng/dl
Female:	Up tp 50 ng/dl


Free Progesterone (As a % of Total Progesterone)

Male:	11-57 %
Female:	5-64 %


Progesterone, Total
Male:	50-50 ng/dl


Female:
Follicular:	10-100 ng/dl
Luteal:	200-1800 ng/dl

Clinical Significance

Progesterone is a Progestin produced primarily from enzymatic metabolism of Pregnenolone. It is enzymatically converted to 17-Hydroxy Progesterone and 11-Deoxycorticosterone. It is secreted by both the gonads and the adrenal glands. It is mostly bound to Cortisol Binding Globulin and Albumin, but a small percentage is present in the "Free" form. This "Free" Progesterone is the bioactive moiety. It is excreted into the urine primarily as "Free" unconjugated Progesterone and as Pregnanediol. Progesterone is responsible for cellular changes in the cervix, vagina, and uterus. Levels are lowest in the follicular phase and increase rapidly following the luteal surge. Increased Progesterone inhibits ovulation. "Free" Progesterone increases greatly during pregnancy reaching about 20% of the total Progesterone concentration at delivery. Measurement of Progesterone can be useful to monitor fertility, corpus luteum function, endometrial development, and be helpful in in-vitro fertilization patients.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.