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Factor VIII Inhibitor Panel
Test Code40083
CPT Codes
85240, 85335
Includes
Factor VIII Inhibitor, EIA Screen and Factor VIII Activity, Clotting
If Factor VIII Inhibitor, EIA Screen is positive, the Nijmegen Assay will be performed at an additional charge (CPT code(s): 85335).
If Factor VIII Inhibitor, EIA Screen is positive, the Nijmegen Assay will be performed at an additional charge (CPT code(s): 85335).
Preferred Specimen
2 mL frozen plasma (x2) collected in a 3.2% sodium citrate (light blue-top) tubes
Minimum Volume
1 mL (x2)
Instructions
Submit a total of 4 mL (2 mL in each of 2 tubes).
Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days
Refrigerated: Unacceptable
Frozen: 14 days
Methodology
See individual tests
Setup Schedule
Set up: Tues, Thurs, Sun; Report available: 4 days
Reference Range
See individual tests
Clinical Significance
The presence of alloantibodies against factor VIII activity is a complication of treatment in hemophilia A, while the presence of autoantibodies may develop spontaneously in patients with acquired Factor VIII-C deficiency. The presence of Factor VIII inhibitor may lead to the neutralization (inactivation) of transfused or endogenous Factor VIII activity. The detection and magnitude of Factor VIII inhibitor is of great importance in the care of these patients. Factor VIII inhibitor EIA assay provides a quick method for the detection of Factor VIII inhibitor with less sample volume.
