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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cortisol, Free, Response to ACTH Stimulation
Test Code16434
CPT Codes
82530 (x3)
Includes
Cortisol, Free: Baseline, 30 minutes, 60 minutes
Preferred Specimen
2 mL serum (x3) collected in a red-top tubes (no gel)
Minimum Volume
2 mL (x3)
Other Acceptable Specimens
2 mL plasma (x3) collected in: EDTA (lavender-top), or PPT potassium (white-top) tubes
Instructions
Draw morning baseline specimen. Administer 250 mcg Cortrosyn. Draw additional specimens at 30 and 60 minutes.
For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory.
This test cannot be collected at a Patient Service Center.
For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory.
This test cannot be collected at a Patient Service Center.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 2 years
Refrigerated: 7 days
Frozen: 2 years
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Serum Separator Tube (SST®)
Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) • Equilibrium Dialysis
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Sun-Thurs; Report available: 4-8 days
Reference Range
Cortisol, Free, Baseline | 8:00-10:00 A.M. | 0.07-0.93 | µg/dL |
Adult | 4:00-6:00 P.M. | 0.04-0.45 | µg/dL |
10:00-11:00 P.M. | 0.04-0.35 | µg/dL | |
Cortisol, Free, 30 min | Not established | ||
Cortisol, Free, 60 min | Not established |
Clinical Significance
Free Cortisol is useful in the detection of patients with Cushing’s syndrome for whom Free Cortisol concentrations are elevated.