Cortisol, Free and Cortisone, 24-Hour Urine with Creatinine

Test Code

37355

CPT Codes
82530, 82542, 82570

Includes

CPT code 81050 may be added at an additional charge for volume measurement

Preferred Specimen

2 mL of 24 hour urine collected in a 24-hour urine container, no preservative

Minimum Volume

0.5 mL

Other Acceptable Specimens

24 hour urine collected in a leak-proof urine container, with 25 mL 6N HCl, or 10 mL conc. glacial acetic acid, or 10 g Boric acid

Instructions

Collect urine with 10 grams of boric acid or keep urine refrigerated during collection if preservative is not used. Record 24-hour urine volume on test requisition and urine vial.

Reference ranges do not apply to random urine samples.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 90 days

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Fri; Report available: 3-7 days

Reference Range

Cortisol, Free, 24-Hr Urine

Adult	4.0-50.0 mcg/24 hrs
Pediatric
1-4 Years	0.9-8.2 mcg/24 hrs
5-9 Years:	1.0-30.0 mcg/24 hrs
10-13 Years	1.0-45.0 mcg/24 hrs
14-17 Years:	3.0-55.0 mcg/24 hrs

Cortisone, 24-Hr Urine

Adult

23-195 mcg/24 hrs

Creatinine, 24-Hour Urine

<3 Years	Not established
3-8 Years	0.10-0.80 g/24 hours
9-12 Years	0.20-1.40 g/24 hours
13-17 Years	0.40-1.90 g/24 hours
>17 Years	0.50-2.15 g/24 hours

Clinical Significance

Urinary free cortisol is useful in the detection of patients with Cushing's syndrome for whom free cortisol concentrations are elevated

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.