Borrelia Species DNA, Real-Time PCR, with Reflexes, Blood

Test Code

39219

CPT Codes
87801

Includes

If Borrelia Species DNA, Real-Time PCR, Blood is Detected, then Borrelia burgdorferi DNA, Qualitative Real-Time PCR, Miscellaneous and Borrelia miyamotoi DNA, Real-Time PCR, Miscellaneous will be performed at an additional charge (CPT Code(s): 87476, 87478).

Preferred Specimen

3 mL whole blood collected in an EDTA (lavender top) tube or ACD (yellow-top) tube

Minimum Volume

0.5 mL

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Lithium heparin (green-top) tube • Sodium heparin (green-top) tube

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 2-3 days

Reference Range

Not detected

Clinical Significance

The diagnosis of Borreliosis is most often made by clinical examination combined with evidence of tick bite or exposure in endemic areas. Amplification of Borrelia genomic DNA from blood, fluids or tissues can support the diagnosis. This test detects Borrelia species which are associated with either Lyme disease (Borrelia burgdorferi), or relapsing fever (Borrelia miyamotoi).
A positive result for Borrelia species but negative result for Borrelia burgdorferi and Borrelia miyamotoi, may indicate that infection is caused by other Borrelia species. A negative result for Borrelia species does not rule out Borrelia infection and appropriate serology testing may be clinically warranted.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.