Follicular Lymphoma, BCL-2/JH t(14;18), Real-Time PCR, Cell-based

Test Code

15007

CPT Codes
81278<br />

Preferred Specimen

5 mL whole blood or bone marrow collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Other Acceptable Specimens

Formalin-fixed, paraffin-embedded tissue block • Fresh (unfixed) tissue submitted in RPMI or in formalin fixative

Instructions

Fresh frozen tissue (acceptable): stable for approximately 1 year at -70ºC.

Fresh (unfixed) tissue in RPMI or tissue in formalin fixative (acceptable): Ship at room temperature or refrigerated. Identify what fixative or medium is used and write it on the sample container.

Transport Temperature

Room temperature

Specimen Stability

Whole blood and bone marrow
Room temperature: 7 days
Refrigerated: 14 days
Frozen: Unacceptable

Paraffin Block
Room temperature: Indefinitely
Refrigerated: Unacceptable
Frozen: Unacceptable

Fresh Tissue
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen (-70°C): 1 year

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Tues, Sat; Report available: 4 days

Reference Range

Negative

Clinical Significance

Follicular lymphoma is the most common type of non-Hodgkin's lymphoma in the United States. Up to 90% of follicular lymphomas carry BCL-2/JH t(14;18) (q32;Q21) chromosome translocation. The presence of the BCL-2 translocation has been associated with poor prognosis in one group of diffuse large-cell and follicular lymphoma patients. Analysis of BCL-2 translocations provides an important marker for detection and monitoring of malignancy during patient treatment and follow up.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.