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Anaerobic Culture II
Test Code15871
CPT Codes
87075
Preferred Specimen
0.5 mL body fluid or 0.5 grams tissue submitted in an anaerobic transport system
Abscess or deep wound, tissue, normally sterile body fluids other than blood, transtracheal aspirations, bronchoscopic specimens obtained by the double lumen technique, suprapubic urine aspirates obtained from bladder, nephrostomy tube or suprapubic catheterization, genital specimens from these sites only (cul-de-sac aspiration, culdocentesis, percutaneous transfundal aspiration, placenta, fallopian tube, intrauterine devices, surgical specimens), exudates, aspirated pus from deep wound or abscesses and tympanocentesis fluid.
Abscess or deep wound, tissue, normally sterile body fluids other than blood, transtracheal aspirations, bronchoscopic specimens obtained by the double lumen technique, suprapubic urine aspirates obtained from bladder, nephrostomy tube or suprapubic catheterization, genital specimens from these sites only (cul-de-sac aspiration, culdocentesis, percutaneous transfundal aspiration, placenta, fallopian tube, intrauterine devices, surgical specimens), exudates, aspirated pus from deep wound or abscesses and tympanocentesis fluid.
Minimum Volume
0.5 mL or 0.5 g
Other Acceptable Specimens
Swab submitted in anaerobic transport system or a swab submitted in ESwab (See Instructions)
Instructions
Tissues and fluids are superior to a swab specimen.
If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain or submit one anaerobic transport swab with one air dried smear or one eSwab (white cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.
Do not refrigerate or freeze.
Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe.
If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain or submit one anaerobic transport swab with one air dried smear or one eSwab (white cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.
Do not refrigerate or freeze.
Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe.
Transport Temperature
Room temperature
Specimen Stability
Body Fluid or Tissue
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Swab
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Swab
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Specimens not submitted in anaerobic transport media/device • Expired transport media • Specimens collected from the following sites: throat, nasopharyngeal, sputum or bronchoscopic • Specimens not collected by double lumen technique • Feces and rectal swabs (except for clostridium difficile culture) • Urine-voided or catheter • Vaginal • Cervical • Specimens from sites contaminated with intestinal contents (colostomy sites, draining pilonidal sinus or traumatic perforation of bowel) • Superficial wounds • External ear • External eye
Methodology
Culture
Setup Schedule
Set up: Daily; Report available: 6 days
Reference Range
See Laboratory Report
