|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Culture, Anaerobic Bacteria with Gram Stain [4469X]
Test Code13126
CPT Codes
87075, 87205
Includes
If culture is positive, identification will be performed at an additional charge (CPT code(s): 87076 or 87143 or 87140 or 87149). Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87185 or 87186).
Preferred Specimen
3 grams tissue or abscess, or wound specimen, or 3 mL body fluid collected in an anaerobic transport gel vial - See Instructions
Minimum Volume
1 gram or 1 mL
Other Acceptable Specimens
(2 swabs) anaerobic transport swabs collected in Amies gel (blue-cap) or ESwab (white-cap) - See Instructions
Instructions
3 mL or 3 grams (1 mL or 1 gram) or 2 swabs, abscess or deep wound, or tissue, or normally sterile body fluids other than blood, bronchoscopic specimens obtained by the Double Lumen Brush technique, suprapubic urine aspirates obtained from bladder, Nephrostomy tube or suprapubic catheterization or aspirated pus from deep wound or abscess or tympanocentesis fluid, genital specimens from these sites:
Only -cul-de-sac aspiration -culdocentesis -percutaneous transfundal aspiration, fallopian tube, surgical specimens, exudates collected in a Anaerobic transport gel vial for fluid and tissue specimens or Anaerobic transport swab with Amies gel (blue-cap) or ESwab (white-cap).
Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain, or submit one anaerobic transoprt swab with one air dried smear, or one eSwab (white-cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director. Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe. Purulent sterile body fluids not submitted in an anaerobic transport tube may be accepted for culture if received within 8 hours of collection.
Only -cul-de-sac aspiration -culdocentesis -percutaneous transfundal aspiration, fallopian tube, surgical specimens, exudates collected in a Anaerobic transport gel vial for fluid and tissue specimens or Anaerobic transport swab with Amies gel (blue-cap) or ESwab (white-cap).
Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for Gram stain, or submit one anaerobic transoprt swab with one air dried smear, or one eSwab (white-cap) for both culture and Gram stain. Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director. Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe. Purulent sterile body fluids not submitted in an anaerobic transport tube may be accepted for culture if received within 8 hours of collection.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Anaerobic transport media
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable
ESwab or Amies gel swab
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable
ESwab or Amies gel swab
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens not in anaerobic transport medium • Expired transport media • Throat, nasopharyngeal, sputum or bronchoscopic specimens • Specimens not collected by Double Lumen Brush technique • Stool and rectal swabs • Urine-voided or catheter • Vaginal and cervical • Specimens from sites contaminated with intestinal contents • Superficial or fresh wounds • External ear • External eye • Intrauterine device • Tracheal aspirates
Methodology
Direct Microscopy • Bacterial Culture • Anaerobic Isolation and Identification Procedures • Phenotypic • Matrix Assisted Laser Desorption Ionization-Time of Flight • Sequencing
Setup Schedule
Set up: Daily; Report available: 5-8 days
Reference Range
No growth
Clinical Significance
Anaerobic bacteria cause a variety of human infections. The results of anaerobic cultures assist the clinician with diagnosis and treatment of patients with anaerobic infections.
