Clinical Trial Cardio IQ Vitamin D 25 LC/MS/MS

Test Code

991735

CPT Codes
82306<br>Restricted Client Code

Preferred Specimen

0.5 mL serum

Patient Preparation
Fasting preferred, but not required.

Minimum Volume

0.3 mL

Instructions

Collect blood in a standard red-top serum vacutainer tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.

Alternatively, collect blood in a serum separator tube, allow to clot at room temperature, centrifuge and remove from the gel within 48 hours.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 21 days
Refrigerated: 21 days
Frozen -20° C: 21 days
Frozen -70° C: 18 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Grossly hemolyzed and lipemic samples • Samples outside of stability • Icteric samples • Serum not separated from SST gel or clot within 48 hours

Methodology
Liquid Chromatography/Tandem Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Sat; Report available: 2 days

Reference Range

Vitamin D, 25-OH, Total: 30-100 ng/mL
Vitamin D, 25-OH, D3: Not established
Vitamin D, 25-OH, D2: Not established

For more information on this test, go to http://education.questdiagnostics.com/faq/25-OHVitaminD

Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor abosrption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia.

This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D 3 - the endogenous form of the vitamin and 25OH D 2 - the analog form used to treat 25OH Vitamin D 3 deficiency.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.