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Cortisol Response to ACTH (baseline, 30 minutes and 60 minutes)
Test Code94527
CPT Codes
82533 (x3)<br>Limited Access Code
Preferred Specimen
1 mL serum (x3) collected in red-top tubes (no gel)
Minimum Volume
0.1 mL (x3) serum
0.2 mL (x3) plasma
0.2 mL (x3) plasma
Other Acceptable Specimens
1 mL plasma (x3) collected in EDTA (lavender-top) tubes
Instructions
For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.
Draw morning baseline specimen. Administer 250 mcg Cortrosyn. Draw additional specimens at 30 and 60 minutes.
Please note: Cortrosyn (Cosyntropin) not supplied by the laboratory. This test cannot be collected at a patient service center.
Draw morning baseline specimen. Administer 250 mcg Cortrosyn. Draw additional specimens at 30 and 60 minutes.
Please note: Cortrosyn (Cosyntropin) not supplied by the laboratory. This test cannot be collected at a patient service center.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year
Refrigerated: 21 days
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly hemolyzed • Serum separator tubes (SST)
Methodology
Liquid Chromatography/Tandem Mass Spectrometry
Setup Schedule
Set up: Mon-Sat; Report available: 3-7 days
Reference Range
Cortisol Baseline: 5-21 mcg/dL
Cortisol 30 min: 14-36 mcg/dL
Cortisol 60 min: 14-41 mcg/dL
Cortisol 30 min: 14-36 mcg/dL
Cortisol 60 min: 14-41 mcg/dL
Clinical Significance
The cortisol response to infusion of synthetic ACTH is useful in diagnosing patients with adrenal insufficiency (Addison's disease).
