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HIV-1 RNA, Quantitative, Real-Time PCR
Test Code93758
CPT Codes
87536<br /> Restricted Client Code
Preferred Specimen
3 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
PPT potassium EDTA (white-top) tube
Instructions
Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. If shipping frozen, transfer plasma to a leakproof transport tube, then freeze prior to shipment. Follow manufacturer's instructions for collection tube handling.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 6 days
Frozen: 42 days
Refrigerated: 6 days
Frozen: 42 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Plasma received frozen in the plasma preparation tube (PPT) • Serum • Whole blood greater than 24 hours old • Frozen whole blood
Methodology
Real-Time PCR
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Reference Range
See Laboratory Report
Clinical Significance
This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.
