HPV Genotypes 16 and 18, Cervical

Test Code

93255

CPT Codes
87625<br>Restricted Client Code

Preferred Specimen

ThinPrep®: 3 mL PreservCyt® fluid collected in a PreservCyt® liquid based pap smear collection vial, or
SurePath™: 2 mL SurePath fluid collected in a SurePath™ liquid-based pap smear vial

Minimum Volume

ThinPrep®: 1.5 mL
SurePath™: 1 mL

Instructions

Collect the sample as usual according to the manufacturer's instructions.

Transport Temperature

Room temperature

Specimen Stability

ThinPrep®
Room temperature: 90 days
Refrigerated: 90 days
Frozen: Unacceptable

SurePath™
Room temperature: 28 days
Refrigerated: 90 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Cervical swabs in Digene® HC cervical sampler • Unprocessed Cytyc® media without cervical brush/broom • Swabs • Digene® vials • SurePath™ pellet • Samples treated with acetic acid • Vaginal sources • Biopsy

Methodology
Real-Time PCR

Setup Schedule

Set up: Tues, Thurs, Sat; Report available: 1-3 days

Reference Range

HPV 16: Not detected
HPV 18: Not detected

Clinical Significance

Persistent infection with high-risk HPV types increases a woman's risk for progression to dysplasia or cervical cancer. The rate of progression or degree of risk depends on the HPV type. Of all various high-risk types, HPV Types 16 and 18 cause more than 60-65% of cervical cancers. This test will identify the presence or absence of HPV Type 16 DNA and /or Type 18 DNA. Knowledge of the HPV genotype will help physicians diagnose the type of HPV infection and its risk of developing into dysplasia or cervical cancer.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.