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Fibrinolysis and von Willebrand Panel (NCCAPS)
Test Code902506
CPT Codes
85240, 85246, 85362, 85379, 85610, 85730<br>Restricted Client Code
Includes
10142 D-Dimer, Quantitative
10143 Fibrinogen Degradation Products (FDP)
10144 Prothrombin Time with INR
10148 Von Willebrand Factor Antigen
10149 Partial Thromboplastin Time, Activated
10150 Factor VIII Activity, Clotting
10143 Fibrinogen Degradation Products (FDP)
10144 Prothrombin Time with INR
10148 Von Willebrand Factor Antigen
10149 Partial Thromboplastin Time, Activated
10150 Factor VIII Activity, Clotting
Preferred Specimen
4.6 mL plasma collected in 3.2% sodium citrate (light blue-top) tube
Minimum Volume
2.3 mL
Instructions
Please submit a separate, frozen vial for each special coagulation assay ordered.
Platelet-Poor Plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid WBS/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice.
A completely filled tube is necessary because the correct ratio of blood to citrate is critical (9:1). Mix by gentle inversion 3-4 times. Do not uncap.
Storage of whole blood at refrigerated temperatures.
Whole blood: The tubes should be at 100% + / -10% fill volume in order to obtain the correct ratio.
Note: for patients receiving Hemilbra (aka emicizumab), the Factor VIII activity clotting method is not recommended. Hemilbra will cause falsely elevated Factor VIII activity values. A chromogenic Factor VIII Activity method is recommended.
Platelet-Poor Plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid WBS/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (< 10,000/mcL). Freeze immediately and ship on dry ice.
A completely filled tube is necessary because the correct ratio of blood to citrate is critical (9:1). Mix by gentle inversion 3-4 times. Do not uncap.
Storage of whole blood at refrigerated temperatures.
Whole blood: The tubes should be at 100% + / -10% fill volume in order to obtain the correct ratio.
Note: for patients receiving Hemilbra (aka emicizumab), the Factor VIII activity clotting method is not recommended. Hemilbra will cause falsely elevated Factor VIII activity values. A chromogenic Factor VIII Activity method is recommended.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days
Refrigerated: Unacceptable
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia • Clotted specimen • Improper blood to citrate ratio • Plasma received room temperature • Plasma received room temperature or refrigerated • High hematocrits • Received thawed
Methodology
See individual assays
Setup Schedule
Set up and Report available: See individual assays
Reference Range
See Laboratory Report
