HCV with Reflex to HIV, HBV Core, HBV Surface, HBV Surface Qualitative

If Hepatitis C Viral RNA, Quantitative Real-Time PCR viral load is Positive, then HIV 1/2 Ag/Ab, HBcAb, HBsAg, HBsAb will be added at an additional charge (CPT code(s): 87389, 86704, 87340, 86706).

If HIV Ag, Ab, 4th Generation Screen is Reactive, then HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).

If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 and HIV-2 RNA, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87535, 87538).

For Reflexes:
Tube #1 for test code 91431:
4 mL (2 mL minimum) serum collected in serum separator tube (SST) or red-top tube (no gel)
Serum: Collect in a serum separator tube (SST) or red-top tube (no gel). The tube should be centrifuged after clotting.
Spun SSTs may be submitted at room temperature or refrigerated unopened without transfer to a plastic screw-cap vial.
Red-top tube (no gel) serum should be transferred to a plastic screw-cap vial and submitted for testing.

Plasma: Collect in EDTA (lavender top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma, and submit for testing.

Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.

and
Tube #2 for test code(s): 501, 498, and 499:
4 mL (2 mL minimum) serum in serum separator tube (SST) or red-top tube (no gel)

and
Tube #3 for Screening Test Code 35645 - 2 mL (1 mL minimum) plasma collected in EDTA (lavender-top) tube