|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
AFB Identification
Test Code901631
CPT Codes
87150, 87153 (x3)<br>Restricted Client Code
Preferred Specimen
Pure culture of isolate submitted on a slant
Other Acceptable Specimens
Pure culture of isolate submitted in 2 mL appropriate broth culture media
Instructions
Submission of pure isolate is required. Specify the type of media used.
This test code is for identification of a positive AFB culture. Indicate specimen source of organism and any other pertinent information such as the growth rate of the organism, growth temperature, DNA probe results, or preliminary identification. The laboratory should be notified when the presence of M. genavense, M. haemophilum and other fastidious mycobacteria are suspected, as these organisms will not grow on media routinely used for subcultures.
If colony morphology on a solid medium resembles Mycobacterium tuberculosis (MTB) complex, a line probe assay to detect M. tuberculosis complex will be performed. If positive, first-line drug antimicrobial susceptibility testing (AST; MTB2) will be performed on all initial isolates. The laboratory will provide at no charge a subculture to the respective state laboratory for genotyping upon request.
A line probe assay to rule out the presence of M. tuberculosis will be performed on ALL liquid cultures submitted without preliminary identification unless a clinical history indicating nontuberculous mycobacterial (NTM) infection is provided or indicated on the requisition that M. tuberculosis has been ruled out.
All other mycobacteria will be identified using DNA sequencing (i.e., rpoB gene and 16S rRNA gene sequencing).
Additional distinction within the group Mycobacterium abscessus can be made using molecular assays for erm(41) and hsp65 genes, which will be ordered after preliminary identification of M. abcessus group. This will also be performed if M. abcessus group is identified by our laboratory.
There is an additional charge for purification/isolation of cultures containing a non-acid-fast contaminant, or more than one mycobacterial species
This test code is for identification of a positive AFB culture. Indicate specimen source of organism and any other pertinent information such as the growth rate of the organism, growth temperature, DNA probe results, or preliminary identification. The laboratory should be notified when the presence of M. genavense, M. haemophilum and other fastidious mycobacteria are suspected, as these organisms will not grow on media routinely used for subcultures.
If colony morphology on a solid medium resembles Mycobacterium tuberculosis (MTB) complex, a line probe assay to detect M. tuberculosis complex will be performed. If positive, first-line drug antimicrobial susceptibility testing (AST; MTB2) will be performed on all initial isolates. The laboratory will provide at no charge a subculture to the respective state laboratory for genotyping upon request.
A line probe assay to rule out the presence of M. tuberculosis will be performed on ALL liquid cultures submitted without preliminary identification unless a clinical history indicating nontuberculous mycobacterial (NTM) infection is provided or indicated on the requisition that M. tuberculosis has been ruled out.
All other mycobacteria will be identified using DNA sequencing (i.e., rpoB gene and 16S rRNA gene sequencing).
Additional distinction within the group Mycobacterium abscessus can be made using molecular assays for erm(41) and hsp65 genes, which will be ordered after preliminary identification of M. abcessus group. This will also be performed if M. abcessus group is identified by our laboratory.
There is an additional charge for purification/isolation of cultures containing a non-acid-fast contaminant, or more than one mycobacterial species
Transport Temperature
Room temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Mixed cultures or non-viable organisms
Methodology
ID Kits
Setup Schedule
Set up: Mon-Sat; Report available: Variable, depending on methodology
Reference Range
See Laboratory Report
