T-SPOT®.TB

Test Code

38702

CPT Codes
86481<br>Restricted Client Code

Preferred Specimen

Adults and children ≥ 10 Years:
6 mL whole blood collected in Greiner 9 mL lithium heparin (green-top) tube

Pediatric 2-9 Years of Age:
4 mL whole blood collected in a Greiner 9 mL lithium heparin (green-top) tube

Pediatric < 2 Years of Age:
2 mL whole blood collected in a Greiner 9 mL lithium heparin (green-top) tube

Immunocompromised individuals
Adults and children ≥ 10 Years:
9 mL whole blood collected in a Greiner 9 mL lithium heparin (green-top) tube

Instructions

Collect blood by venipuncture into a Quest-provided Greiner 9 mL lithium heparin (green-top) tube. The tube contents must be inverted (8-10 times) to ensure that the whole blood is mixed thoroughly with the anticoagulant. Specimens must be shipped in accordance to the pre-approved logistics process established for your site. Samples received >39 hours after collection will be rejected. Date and time of draw are required and must be provided.

Note: *****This is a short stability test and requires special handling. Please see above for special collection instructions. If you have any questions, please contact Customer Service 1-800-336-3718 option 1.*****

Transport Container

See instructions

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 39 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Anticoagulants other than lithium heparin or sodium heparin • Hemolyzed • Centrifuged • Samples received > 32 hours after collection • Samples not received in TSPOT ®.TB insulated shipper • Frozen or refrigerated samples

Methodology
Enzyme-Linked Immunosorbent Assay

Setup Schedule

Set up: Daily; Report available: Next day

Reference Range

See Laboratory Report

Clinical Significance

The T-SPOT®.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-y) in the vicinity of T cells in human whole blood collected in lithium heparin. It is intended for use as an aid in the diagnosis of M. tuberculosis infection. The T-SPOT.TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.