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Cytomegalovirus DNA, Qualitative Real-Time PCR
Test Code38682
CPT Codes
87496<br>Restricted Client Code
Preferred Specimen
1 mL whole blood collected in EDTA (lavender-top) tube, or ACD solution A or B (yellow-top) tubes, or
1 mL plasma collected in EDTA (lavender-top) tube, or ACD solution A or B (yellow-top) tubes, or PPT EDTA (white-top) tube, or
1 mL serum or CSF collected in a sterile leak-proof container, or
1 mL bronchoalveolar lavage, or amniotic fluid or supernatant amniotic fluid, or random urine collected in a sterile leak-proof container
1 mL plasma collected in EDTA (lavender-top) tube, or ACD solution A or B (yellow-top) tubes, or PPT EDTA (white-top) tube, or
1 mL serum or CSF collected in a sterile leak-proof container, or
1 mL bronchoalveolar lavage, or amniotic fluid or supernatant amniotic fluid, or random urine collected in a sterile leak-proof container
Minimum Volume
0.3 mL eye fluids • 0.5 mL all other specimens
Other Acceptable Specimens
Minimum: 3 mm fresh tissue collected in a sterile leak-proof container • 1 mL eye fluids collected in a sterile leak-proof container
Transport Temperature
Tissue: Frozen
All other specimens: Refrigerated (cold packs)
All other specimens: Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized specimens • Unspun PPT tube
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
See Laboratory Report
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.
