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Bilirubin, Direct
Test Code38441
CPT Codes
82248<br>Limited Access Code
Preferred Specimen
1 mL serum collected in a serum separator tube - protected from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in a sodium or lithium heparin (green-top) tube, or PST plasma separator tube - protected from light • Serum collected in amber transport tube - protected from light
Instructions
Protect from light by wrapping a spun SST® in foil or transferring serum/plasma to an amber transport vial. Avoid hemolysis.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: 90 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, anticoagulants other than heparin
Methodology
Spectrophotometric
Setup Schedule
Set up: Daily; Report available: Next day
Reference Range
≤ 0.2 mg/dL
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin, associated with obstructive jaundice, is primarily due to the direct (conjugated) fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.