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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
FISH, IGH/CCND1, t(11;14), Technical Component
Test Code37877
CPT Codes
88271 (x2), 88275<br /> Tech Only Code
Preferred Specimen
5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) tube
Minimum Volume
3 mL whole blood • 1 mL bone marrow • 5x5 mm tissue
Other Acceptable Specimens
Whole blood or bone marrow collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • 5x5 mm fresh tissue culture transport media or sterile container with Hanks' or Ringers solution or formalin-fixed paraffin-embedded tissue block
Instructions
Methodology: FISH (fluorescence in situ hybridization) is performed to detect IGH-CCND1 fusions and rearrangements. 200 interphase nuclei are scored for the probe set and are provided to the client for the purpose of determining rearrangements, gains, and/or losses of the probe region.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Reference Range
Interpretive report
Clinical Significance
This assay for IGH/CCND1 rearrangements such as t(11;14) is seen most often in mantle cell lymphomas and infrequently in chronic lymphocytic leukemia and multiple myeloma. This test is useful for detecting cell populations that may not be mitotically active, that produce metaphase cells of suboptimal morphology, or that have variant translocations.