FISH, MDS/Myeloid Panel, -5/5q-, -7/7q-, +8, 20q-, Technical Component

Test Code

37876

CPT Codes
88271 (x6), 88275 (x3)<br /> Client Restricted - Tech Code

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube

Instructions

Methodology: FISH (fluorescence in situ hybridization) is performed to detect common abnormalities of four probe sets including -5/5q-, -7/7q-, +8, and 20q- that are associated with myelodysplastic syndrome (MDS). 200 interphase nuclei are scored for each probe set and are provided to the client for the purpose of determining gains, losses, and/ or rearrangements of the probe regions.

If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Reference Range

Interpretive report

Clinical Significance

Prognostic investigation in patients with myelodysplasia or other myeloid malignancies. Among the patients with myelodysplastic syndromes (MDS), 45 – 50% have at least one chromosome rearrangement, the most common of which can be detected using this fluorescence in situ hybridization (FISH) panel (-5/5q-, -7/7q-, +8, 20q-). This panel of FISH probes can increase the detection rate of chromosome abnormalities at diagnosis and aid in follow-up testing for clinically significant chromosome abnormalities in MDS. The identification of the specific chromosome regions involved in a patient’s disease may play a significant role in treatment decisions as MDS targeted therapies are developed.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.