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Methotrexate, S
Test Code34197
CPT Codes
80299<br>Restricted Client Code
Preferred Specimen
0.5 mL serum from a serum gel collection container, spun - protected from light
Minimum Volume
0.25 mL
Other Acceptable Specimens
Serum from a red-top, spun - protected from light
Instructions
Include standard and minimum volume, sample type and collection container. 0.5 mL (0.25 mL minimum) Serum from a Serum gel collection container submitted in an Amber vial (protect from light). Acceptable Specimen: Red top
1.) Methotrexate is sensitive to fluorescent light; avoid prolonged exposure of specimen to direct light.
2.) Serum gel tubes should be centrifuged within 2 hours of collection. Protect from light.
3.) Red-top tubes should be centrifuged and serum aliquoted into amber vial within 2 hours of collection.
1.) Methotrexate is sensitive to fluorescent light; avoid prolonged exposure of specimen to direct light.
2.) Serum gel tubes should be centrifuged within 2 hours of collection. Protect from light.
3.) Red-top tubes should be centrifuged and serum aliquoted into amber vial within 2 hours of collection.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 28 days
Refrigerated: 14 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis
Methodology
Immunoassay
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
<0.10 mcmol/L
Clinical Significance
Determining whether methotrexate is being cleared appropriately and verifying that a nontoxic concentration has been attained following therapy
