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Antithrombin III Activity
Test Code30936
CPT Codes
85300<br /> Limited Access Code
Preferred Specimen
1 mL plasma collected in 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Instructions
Please submit a separate vial for each special coagulation assay ordered.
Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the wbc/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the wbc/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis; Received thawed; Received refrigerated
Methodology
Chromogenic Substrate
Setup Schedule
Set up: Mon-Fri; Report available: Next day
Reference Range
1 day-29 days: 41-108 %normal
1 month-2 months: 50-120 %normal
3 months-5 months: 73-120 %normal
> or = 6 months: 80-135 %normal
1 month-2 months: 50-120 %normal
3 months-5 months: 73-120 %normal
> or = 6 months: 80-135 %normal
