Factor V Activity, Clotting

Test Code
30745


CPT Codes
85220<br>Limited Access Code

Preferred Specimen
1 mL platelet-poor plasma collected in 3.2% sodium citrate (light blue-top) tubes


Minimum Volume
0.5 mL


Instructions
Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.


Transport Container
Plastic vial


Transport Temperature
Frozen


Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received thawed


Methodology
Photometric Clot Detection

Setup Schedule
Set up: Mon-Fri; Report available: Next day


Reference Range
See Laboratory Report




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.