Factor VII R353Q Mutation Analysis

Test Code

16180

CPT Codes
81400

Preferred Specimen

5 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

3 mL

Other Acceptable Specimens

ACD solution A or B (yellow-top) tube • Lithium heparin (green-top) tube • Sodium heparin (green-top) tube

Instructions

Whole blood: Normal phlebotomy procedure. Specimen stability is crucial. Store and ship room temperature immediately. Do not freeze.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 8 days
Refrigerated: 8 days
Frozen: Do not freeze

Methodology
Polymerase Chain Reaction (PCR) • Single Nucleotide Primer Extension

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Thurs; Report available: 8 days

Reference Range

See Laboratory Report

Clinical Significance

Factor VII is a serine protease found in plasma and is one of the vitamin K-dependent coagulation factors, along with prothrombin (factor II), factors IX and X, and proteins C and S. Factor VII is synthesized principally in the liver and is secreted as a single-chain glycoprotein of apparent M, 48,000. Cleavage of human factor VII to factor VIIa, a two-chain form held together by disulfide bonds, results in a 20-to 25-fold increase in enzyme activity. This cleavage can be affected by a number of activated coagulation factors, including factors XIIa, Xa, IXa, and thrombin. In the presence of tissue factor and calcium ions, factor VIIa converts factor X to factor Xa in the initiating reaction of the extrinsic coagulation pathway. The R353Q allele may confer protection against thrombosis.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.