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Pyruvate Kinase Enzyme Activity, Blood
Test Code39855
CPT Codes
84220
Preferred Specimen
6 mL whole blood collected in an ACD solution B (yellow-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
Whole blood collected in an EDTA (lavender-top) tube
Instructions
Send specimen in original tube. Do not transfer blood to other container.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 20 days
Frozen: Unacceptable
Refrigerated: 20 days
Frozen: Unacceptable
Methodology
Kinetic Spectrophotometry
Setup Schedule
Monday-Saturday Morning
Report available: Next Day
Report available: Next Day
Reference Range
≥12 months of age: 5.5-12.4 U/g Hb
Reference Values have not been established for patients who are less than 12 months of age.
Reference Values have not been established for patients who are less than 12 months of age.
Clinical Significance
Evaluation of nonspherocytic hemolytic anemia.
Evaluation of neonatal anemia or jaundice.
Evaluation of unexplained noninfectious hepatic failure.
Evaluation of iron overload.
Evaluation of unusually severe hemoglobin S trait.
Evaluation of unusually severe glucose 6-phosphate dehydrogenase deficiency. Investigating families with pyruvate kinase deficiency to determine inheritance patten and for genetic counseling.
Evaluation of neonatal anemia or jaundice.
Evaluation of unexplained noninfectious hepatic failure.
Evaluation of iron overload.
Evaluation of unusually severe hemoglobin S trait.
Evaluation of unusually severe glucose 6-phosphate dehydrogenase deficiency. Investigating families with pyruvate kinase deficiency to determine inheritance patten and for genetic counseling.
