FISH, ALL, TCF3/PBX1, t(1:19)(q23.3:p13.3)

Test Code

90511

CPT Codes
88271 (x2), 88275

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube or sodium heparin lead-free (tan-top) tube • 5x5mm fresh tumor tissue in transport media

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Note: 100-300 interphase cells are microscopically analyzed for probe signal patterns indicating t(1;19) (q23.3;p13.3).
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Container

Sodium heparin (green-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Reference Range

See Laboratory Report

Clinical Significance

FISH testing with the TCF3-PBX1 dual fusion dual color probe allows detection of balanced and unbalanced translocations involving 1q23.3 (PBX1) and 19p13.3 (TCF3). This translocation has been observed in acute lymphoblastic leukemia with a precursor-B immunophenotype. Furthermore, a variant translocation, involving 17q22 (HLF) and 19p13.3 (TCF3) can be detected with this probe.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.