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FISH, PDGFRB, 5q33.1
Test Code90510
CPT Codes
88271 (x2), 88275
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Other Acceptable Specimens
Bone marrow collected in: Transport medium, sodium heparin (royal blue-top) tube, or sodium heparin lead free (tan-top) tube • Whole blood collected in: Sodium heparin (royal blue-top) tube or sodium heparin lead free (tan-top) tube • 5x5 mm fresh tumor tissue in transport medium • 5x5 mm tumor tissue in formalin-fixed paraffin-embedded tissue block
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Note: 100-300 interphase cells are microscopically analyzed for probe signal patterns indicating rearrangement of 5q33.1.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Note: 100-300 interphase cells are microscopically analyzed for probe signal patterns indicating rearrangement of 5q33.1.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Transport Temperature
Room temperature
Specimen Stability
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Reference Range
Interpretive report
Clinical Significance
FISH testing with the PDGFRB probe allows detection of translocations involving PDGFRB and another chromosome region (usually a tyrosine kinase coding gene). Fusion of PDGFRB with a tyrosine kinase coding gene leads to kinase activation.
