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FISH, Synovial Sarcoma, SYT, 18q11.2 Rearrangement
Test Code19773
CPT Codes
88275, 88271 (x2)
Preferred Specimen
3 mL bone marrow submitted in culture transport media, or 5x5mm tissue in formalin-fixed paraffin-embedded tissue block, or 5x5mm fresh biopsy collected in sterile leak-proof container with culture medium and antibiotics
Minimum Volume
1 mL bone marrow
Other Acceptable Specimens
Bone marrow collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.
Note: Principle of test: 100 - 300 interphase cells are microscopically analyzed for probe signal patterns, indicating presence, absence, or rearrangement of the SYT region of chromosome 18q11.2. This test will detect, but not differentiate, the subtypes of synovial sarcoma.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.
Note: Principle of test: 100 - 300 interphase cells are microscopically analyzed for probe signal patterns, indicating presence, absence, or rearrangement of the SYT region of chromosome 18q11.2. This test will detect, but not differentiate, the subtypes of synovial sarcoma.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Sun-Fri; Report available: 5 days
Reference Range
See Laboratory Report
Clinical Significance
Chromosomal rearrangements of the SYT gene, located at 18q11.2, are common among synovial sarcoma soft tissue tumors, namely t(X;18)(p11.2;q11.2). Whereas there is no variation on the 18q11.2 breakpoint, 2 breakpoints in close proximity on the X chromosome identify the monophasic and biphasic subtypes.
