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FISH, Locus-specific Probe (x1)
Test Code17355
CPT Codes
88271, 88275
Includes
This test code is used for ordering interphase analysis of 100-300 cells by fluorescence in situ hybridization (FISH) using one probe. The probe must be specified in the test order. Additional analytes may be available. Contact performing laboratory.
If one or more FISH tests are ordered on a specimen without chromosome analysis, a tissue culture test code will be added at an additional charge.
If results are not possible, the test order may be canceled and replaced by a Cytogenetics Communicaton.
If one or more FISH tests are ordered on a specimen without chromosome analysis, a tissue culture test code will be added at an additional charge.
If results are not possible, the test order may be canceled and replaced by a Cytogenetics Communicaton.
Preferred Specimen
5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green, royal blue, or lead-free tan-top) tube, or
5X5 mm fresh tissue collected in culture transport media or sterile container with Hanks' or Ringers solution, or formalin-fixed, paraffin-embedded tissue, or
5 mL amniotic fluid collected in a sterile container, or
5 mg chorionic villus sample collected in culture media
5X5 mm fresh tissue collected in culture transport media or sterile container with Hanks' or Ringers solution, or formalin-fixed, paraffin-embedded tissue, or
5 mL amniotic fluid collected in a sterile container, or
5 mg chorionic villus sample collected in culture media
Minimum Volume
1 mL whole blood or bone marrow • 1 mL amniotic fluid • 5 mg cvs
Instructions
Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
3-5 mL whole blood or 1-3 mL bone marrow collected in sodium heparin tube, 5 mL Amniotic fluid collected in sterile container, 5 mg CVS in culture media, 5 X 5 mm Fresh tissue in sterile container, formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
3-5 mL whole blood or 1-3 mL bone marrow collected in sodium heparin tube, 5 mL Amniotic fluid collected in sterile container, 5 mg CVS in culture media, 5 X 5 mm Fresh tissue in sterile container, formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Reference Range
See Laboratory Report
Clinical Significance
This FISH (fluorescence in situ hybridization) assay is performed using any single probe.